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Left: schematic of equilibration setup. The crystal (green) sits in a 40 µl pot with two tubes used for the introduction of new solution and simultaneous withdrawal of the existing solution. Right: detail of the actual system. The crystal sits in the PCR tube cap, which sits on a glass cover slip. The blue housing holding the cover slip and tubing sits on a platform (red), which includes a port for the microscope.

Journal: Acta Crystallographica Section F: Structural Biology Communications

Article Title: Automated gradient equilibration of macromolecular crystals to new solution conditions

doi: 10.1107/S2053230X25008398

Figure Lengend Snippet: Left: schematic of equilibration setup. The crystal (green) sits in a 40 µl pot with two tubes used for the introduction of new solution and simultaneous withdrawal of the existing solution. Right: detail of the actual system. The crystal sits in the PCR tube cap, which sits on a glass cover slip. The blue housing holding the cover slip and tubing sits on a platform (red), which includes a port for the microscope.

Article Snippet: Next, the crystal(s) are prepared as follows. (i) A crystal pot is prepared by using a razor blade to separate a single transparent PCR tube cap from a strip of caps (for example catalog No. 27-110, Genesee Scientific, Morrisville, North Carolina, USA) and inverting the cap.

Techniques: Microscopy

Overview of the complete system, showing the Raspberry Pi touch screen, red housing, the three syringe pumps and the blue housing which holds the PCR tube cap. The first two syringe pumps are loaded with syringes. Not visible in the image are the microscope, Arduino microcontroller with motor control shield, microscope (mounted below the red platform) and the Raspberry Pi (mounted on the back side of the touch screen). Each syringe pump is comprised of a black stepper motor coupled to a lead screw that moves the syringe plunger up and down via the white housing, which is threaded on the lead screw.

Journal: Acta Crystallographica Section F: Structural Biology Communications

Article Title: Automated gradient equilibration of macromolecular crystals to new solution conditions

doi: 10.1107/S2053230X25008398

Figure Lengend Snippet: Overview of the complete system, showing the Raspberry Pi touch screen, red housing, the three syringe pumps and the blue housing which holds the PCR tube cap. The first two syringe pumps are loaded with syringes. Not visible in the image are the microscope, Arduino microcontroller with motor control shield, microscope (mounted below the red platform) and the Raspberry Pi (mounted on the back side of the touch screen). Each syringe pump is comprised of a black stepper motor coupled to a lead screw that moves the syringe plunger up and down via the white housing, which is threaded on the lead screw.

Article Snippet: Next, the crystal(s) are prepared as follows. (i) A crystal pot is prepared by using a razor blade to separate a single transparent PCR tube cap from a strip of caps (for example catalog No. 27-110, Genesee Scientific, Morrisville, North Carolina, USA) and inverting the cap.

Techniques: Microscopy, Control